DOI: http://dx.doi.org/10.18203/2349-2902.isj20210926

Microbiological and antibiotic profile of osteomyelitis in tertiary care hospital

Vijayakumar A. B., Yerriswamy Parvatha Reddy, Suphala B., Ananya Gopalakrishnan, Vinod Kumar C. S.

Abstract


Background: Osteomyelitis has been continuing as the most important cause of morbidity among patients with bone infections. Constant change in the trend of organisms involved and resistance pattern has made management of osteomyelitis cases difficult. With this background present study aimed to look for the changing trends of microorganisms involved in osteomyelitis and their antimicrobial susceptibility pattern.  

Methods: A total of 100 cases studied over a period of two years. Samples collected were processed by standard microbiological techniques and antimicrobial testing was done as per the clinical and laboratory Standards Institute guidelines.

Results: Among 100 cases, 62 were males and 38 were females, with the mean age of all the patient was 51.6±12.32 years. 64% of the patient were diabetic patients. Long bones are most involved with trauma (45%) as risk factor. Staphylococcus aureus (24.2%) predominant pathogen isolated followed by Pseudomonas aeruginosa (21.2%) and Acinetobacter baumannii (16.7%). Antibiotic sensitivity testing of gram positive organisms showed hundred percent sensitivity to vancomycin and gram negative bacteria showed highest sensitivity to cefoperazone+sulbactam, piperacillin+tazobactam, meropenem and imipenem.  

Conclusions: Osteomyelitis caused by methicillin resistant staphylococcus aureus and carbapenem resistance gram negative bacteria is a serious concern. Since multidrug resistant strains have emerged in osteomyelitis cases, emphasis should be given for hygiene and targeted antibiotherapy.  

 


Keywords


Osteomyelitis, Microbiological profile, Antibiogram

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References


de Carvalho VC, de Oliveira PRD, Dal-Paz K, de Paula AP, Felix CDS, Lima LLM. Gram-negative osteomyelitis: clinical and microbiological profile. Braz J Infect Dis. 2012;16:63-7.

Zuluaga AF, Galvis W, Vesga O. Etiologic diagnosis of chronic osteomyelitis. Arch Intern Med 2006;166:95-100.

Abid AS, Ehan AH, Yonis AR. Epidemiological and bacteriological study of chronic osteomyelitis. Tikrit Medic J. 2008;14(1):59-62.

Reza M, Shahab F, Jalal K, Farhad. Epidemic assessment of bacterial agents in osteomyelitis and their antibiotic resistance pattern determination. J Biologic Sci. 2008;8(2):478-81.

Rao PS, Beena VK, Rao PS, Shivnanda PG. Bacteriological study of bone and joint infections with special reference to anaerobes. Ind J Orthopaed. 1997;31:171-4.

Betty A Forbes, Daniel F. Sahm , Alice S. Weissfeld; Baily & Scott’s Diagnostic Microbiology; 12th edition ; Mosby Inc; 2007.

Collee JG, Miles RS, Watt B. Tests for the identification of bacteria. In: Mackie & McCartney Practical Medical Microbiology. 14th edition. Collee JG, Fraser AG, Marmion BP, Simmons A, editors. Edinburgh: Churchill Livingstone. 2006:131-49

Clinical Laboratory Standards Institutes. Performance Standards for antimicrobial susceptibility testing, XXI International Supplement (M100-S21). Wayne, Pennsylvania, USA: National Commit Clinic Laborat Std. 2016.

Wadekar MD, Anuradha K, Venkatesha D. Chronic Osteomyelitis: Aetiology and Antibiotic Suscept-ibility Pattern. Int J Rec Trends Sci Technol. 2014;9(3):337-40.

Kaur, J, Gulati VL. Bacteriological profile of osteomyelitis with special reference to staphylococcus aureus. Ind J Pract Doc. 2013;4(6).

Sheehy SH, Atkins BA, Bejon P, Byren I, Wyllie D, Athanasou NA, et al. The microbiology of chronic osteomyelitis: prevalence of resistance to common empirical anti-microbial regimens. J Infect. 2010;60:338–43.