Study of morphological spectrum of gallstone and bacteriology of bile in cholelithiasis
DOI:
https://doi.org/10.18203/2349-2902.isj20164402Keywords:
Bacteriological profile, Bile culture, GallstoneAbstract
Background:Biliary calculus disease is one of the most common disorders of gastrointestinal tract. Gall bladder stone are crystalline structure formed by concretion of bile constituents. Aetiology of gall stone is multifactorial. Bile is found to be sterile in normal patient.
Methods: This is a cross sectional study conducted in department of surgery, Lt Lakhiram Agrawal Memorial Medical College, Raigarh, Chhattisgarh, India from October 2015 - October 2016. Total 50 cases were selected and operated by lap /open cholecystectomy were included in this study. During cholecystectomy bile was aspirated and was sent to laboratory for culture. Gallstone retrieved from the specimen was classified based on morphological appearance.
Results:Gallstone disease found to be common in female with ratio of 4:1, with maximum no. of cases found in the age group of 35 to 45 year. Bile culture as positive in 40%. The most common organism was E. coli isolated in 14 cases, followed by Klebseilla in 4 cases.
Conclusions:The frequency of bile infection in this study area was 40%, in which association with mixed type gallstone was found to be significant and the most common bacteria isolated was E. coli.
References
Jhonson DE, Kalpan MM. Pathogenesis and treatment of gallstone. New Eng J Med. 1993;328:412-21.
Small DM. Cholesterol nucleation and growth in gallstone formation. N Eng J Med. 1980;302;1305-11.
Bowen JC, Brenner HI, Ferrnate WA, Maule WF. Gallstone disease Pathophysiology, epidemiology, natural history and treatment option. Medclin North. 1992:76;1143-57.
Cushieri A, Bouchier IAD, The biliary tract. In: Cuschier A, Giles GR, Moosa AR. Essentil surgical practice 2nd ed. Eddinburg Butterworth Heienenanm. 1988:1020-75.
Maki T. Pthogenesis of calcium billuribinate gallstone role of beta glucorinidase and cogulation by inorganic ions, polyelectrolyte and agitation. Ann Surg. 1996;164:90-100.
Johnson DE, Kaplan MM. Pathogenesis and treatment of gallstones. New Engl J Med. 1993;328:412-21.
Prakash A. Chronic cholecystitis and cholelithiasis in India. Int Surg. 1968;49:79-85.
Khurro MS, Mahajan R, Zargar SA, Javid G. Prevalence of biliary tract disease in India: a sonographic study in adult population in Kashmir. Gut. 1989;30:201-05.
Malhotra SL. Epidemiological study of cholelithiasis among railroad workers in India with special reference to causation. Gut. 1968;9:290-5.
Tandon R. Pathogenesis of gallstone in India. Trop Gastroenteral. 1988;9:83-91.
Zuhair R, Bahrani A, Mohammad R, Saleh AL. Prevalence and morphology and chemical composition of gallstones. Iraqi Post Graduate Medical Journal. 2011;10:1.
Ranshoff DF, Gracie WA. The natural history of silent gallstones: the innocent gallstone is not a myth. N Eng J Med. 1982:307:798-800.
Jayanthi V, Palanivelu C, Prasanthi R, Methew S, Srinivasan V. Composition of gallstones in Coimbatore district of Tamil Nadu State. Ind J Gastroenterol. 1998;17:134-5.
Baig SJ, Biswas S, Das S, Basu K, Chattopadhyay G. Histopathological changes in gallbladder mucosa in cholelithiasis: correlation with chemical composition of gallstones. Tropical Gastroenterol. 2002;23:25-7.
Dhar SC, Ansari S, Saha M, Ahmad MM, Rahman MT, Hasa M, et al. Gallstone disease in a rural Bangladesh community. Ind J Gastroenterol. 2001;20:223-6.
Tyagi SP, Tyagi N, Maheshwari V, Ashraf SM, Sahoo P. Morphological changes in diseased gall8bladder: a study of 415 cholecystectomies at Aligarh. J Ind Med Assoc. 1992;90:14-7.
Dubois F, Berthelots G, Levrad H. Cholecystectomy par coeliocopie. Press Med. 1989;18:980.
Yaqin H, Sultan G. Results of culture of gallbladder, bile and gall-stones. J Pak Med Assoc. 1978;28:31-2.
Csendes A, Burdiles P, Maluenda F, Diaz JC, Cseudes P, Mitru N. Simultaneous bacteriological assessment of bile from gallbladder and common bile duct in control subjects and patients with gallstones and common duct stones. Arch Surg. 1996;131:389-94.
Stewart L, Smith AL, Pellegrini AC, Motson RW, Way LW. Pigment gallstones form a composite of bacterial microcolonies and pigment solid. Ann Surg. 1987;206:242-50.
Guo RX, He SG, Shen K. The bacteriology of cholelithiasis. Japan J Surg. 1991;21:606-12.
Ohdan H, Hisashi O, Yamamoto Y. Bacteriological investigation of bile in patient with cholelithiasis. 1993;23:390-5.
Tabata M, Nakayama F. Bacteria and gallstones: etiological significance. Dig Dis Sci. 1981;26:218-24.
Chijiiwa K, Ichimiya H, Kuroki S, Koga A, Nakayama F. Late development of cholangiocarcinoma after the treatment of hepatolithiasis. Surg Gynecol Obstet. 1993;177:279-82.
Sabir O. Infected bile in gallbladder in cholelithiasis dissertation. Karachi: College of Physicians and Surgeon Pakistan. 1998.
Cuschieri A, Bouchier IAD. The biliary tract. In: Cuschieri A, Giles GR, Moosa AR. Essential surgical practice. 2nd ed. Edinburgh: Butterworth-Heienemann. 1988:1020-75.
Ballal M. Bacteriological spectrum of cholecystitis and its antibiogram. Indian J Med Microbiol. 2001;19:212-4.
Matin MA, Kunitomo K, Yada S, Miyoshi Y, Matsumura T, Komi N. Biliary stones and bacteriae in bile study in 211 consecutive cases. Tokushima J Exp Med. 1989;36:11-6.
Vitetta L, Sali A, Moritz V, Shaw A, Carson P, Little P, Elzarka A. Bacteria and gallstone nucleation. Aust N Z J Surg. 1989;59:571-7.
Nakayama F. Intrahepatic calculi: a special problem in east Asia. World J Surg. 1982;6:802-4.
Cetta F. The role of bacteria in pigment gallstone disease. Ann Surg. 1991;213:315-26.
