Effect of vitamin C inhibiting liver fibrosis and lipid peroxidation in biliary obstruction of Wistar rats
Keywords:Vitamin C, Biliary obstruction, Liver fibrosis, Lipid peroxidation, Bile duct ligation
Background: The aim of this study was to evaluate the potential inhibiting effects of vitamin C as an antioxidant against liver fibrosis and lipid peroxidation in the bile duct ligation- induced biliary obstruction of Wistar rats.
Methods: A total of 25 male Wistar albino rats were divided into five groups: sham operated, control (bile duct ligation/BDL) without given vitamin C, BDL with vitamin C 75 mg, BDL with vitamin C 150 mg, and BDL with vitamin C 225 mg. Each group contained 5 animals. Vitamin C was given orally on day 3 after operation and after 14 days following vitamin C administration, all animals were performed laparotomy to obtain liver tissue samples for histopathological investigation of liver fibrosis and blood samples for malondialdehyde (MDA) as lipid peroxidation measurement.
Results: The changes demonstrating hepatic fibrosis including moderate to markedly thickened wall of central veins, localized to diffuse perisinusoidal fibrosis, enlarged portal track, increased number of septa, and thickened width of septa were observed in BDL groups. MDA measurement were also observed in all groups. Treatment of biliary obstruction in BDL groups with vitamin C given orally attenuated liver damage. Both the MDA measurement and histopathologic investigation of hepatic fibrosis were observed to be reduced with the vitamin C treatment.
Conclusions: Our data indicate that vitamin C inhibited liver fibrosis and lipid peroxidation in bile duct ligation-induced biliary obstruction of Wistar rats.
Nakeeb A, Pitt HA. Bile secretion and pathophysiology of biliary tract obstruction. In: Blumgart LH, Jarnagin WR, Belghiti J, Chapman WC, Buchler M, Hann LE, et al. editors. Surgery of the liver, biliary tract, and pancreas. 6th Ed. Philadelphia: Saunders Elsevier; 2017;1;126-8.
Friedman SL. Mechanisms of disease: mechanisms of hepatic fibrosis and therapeutic implications. Nat Clin Pract Gastroenterol Hepatol. 2004;1(2):98-105.
Erenoglu C, Kanter M, Aksu B, Sagiroglu T, Ayvaz S, Actas C, et al. Effect of curcumin on liver damage induced by biliary obstruction in rats. Balkan Med J. 2011;28:352-7.
Kanter M, Yener Z. A rabbit model for liver fibrosis. Scandinavian J Lab Animal Sci. 2001;28:213-23.
Tsai LY, Shih MT, King TL, Hsin SY. Level of plasma lipid peroxides before and after choledocholithotomy in patients with obstructive jaundice. J UOEH. 1992;14(4):261-9.
Shimizu I, Shimamoto M, Saiki K, Furujo M, Osawa K. Lipid peroxidation in hepatic fibrosis. Lipid Peroxidation, Angel Catala, IntechOpen. 2012. Available at: https://www.intechopen.com/books/ lipid-peroxidation/lipid-peroxidation-in-hepatic-fibrosis. Accessed on 06 July 2020.
Chatterjee IB. The history of vitamin C research in India. J Biosci. 2009;34(2):185-94.
Marin JJ, Perez MJ, Serrano MA, Marcias RI. Chemoprotective role of vitamin C in liver disease. In: The Liver. 2018: 139-53.
Yin H, Xu L, Porter NA. Free radical lipid peroxidation: mechanisms and analysis. Chem Rev. 2011;111(10):5944-72.
Levine M, Rumsey SC, Daruwala R, Park JB, Wang Y. Criteria and recommendations for vitamin C intake. J Am Med Assoc. 1999;281(15):1415-23.
Halliwell B, Gutteridge JM. Free radicals in biology and medicine. J Free Radicals Biol Med. 1985;1:331-4.
Rose RC, Bode AM. Biology of free-radical scavengers-an evaluation of ascorbate. FASEB J. 1993;7:1135-42.
Adikwu E, Deo O. Hepatoprotective effect of vitamin C. J Pharmacolog Pharm. 2013;4:84-92.
Bashandy AS, Alwasel SH. Carbon tetrachloride-induced hepatotoxicity and nephrotoxicity in rats: protective role of vitamin C. J Pharmacolog Toxicolog. 2011;6(3):283-92.
Mossa AH, Refaie A, Ramadan A. Effect of exposure to mixture of four organophosphate insecticides at no observed adverse effect level dose on rat liver: the protective role of vitamin C. Res J Environ Toxicol. 2011;5(6):323-35.
Krishnamoorthy P, Sangeetha M. Hepatoprotective effect of vitamin C on sodium nitrite-induced lipid peroxidation in albino rats. Ind J Biochem Biophy. 2008;45(3):206-8.
Su JY, Seyeon B, Jae SK, Jung HY, Eun JC, Jeong HL, et al. Hepatoprotective effect of vitamin C on lithocholic acid-induced cholestatic liver injury in Gulo mice. Euro J Pharmacol. 2015;762:247-55.